In Mato Grosso, OPAN operates in the Juruena River basin with the Apiaká, Rikbaktsa, and Paresi peoples. In another region, in the eastern part of the state, the institution works in the interfluve between the Xingu and Araguaia rivers with the Xavante of Marãiwatsédé. Additionally, in the Middle Araguaia River region, OPAN collaborates with the Tapirapé, Kanela do Araguaia, Maxacali, and Krenak peoples. In recent years, it has also expanded its activities in the Paraguay River Basin, supporting the Chiquitano people in efforts related to land regularization and food security.
The Mato Grosso Program focuses on territorial management through initiatives that promote community-based tourism, Brazil nut collection and commercialization, the development of gardens and backyards, seed collection, and integrated fire management. It also emphasizes territorial monitoring and protection while strengthening Indigenous organizations at both local and state levels.
Actions are carried out in collaboration with Indigenous peoples, organizations, and networks, particularly the Juruena Vivo Network and the Xingu-Araguaia Coalition.